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Abstract: In this paper, the potential of oil palm frond (OPF) juice as an alternative and renewable carbon source to replace the function of glucose to produce volatile compounds by Ceratocystis fimbriata will be reported. The OPF juice was extracted using simple physical separation method. The growths of fungi were monitored by using cell dry weight (CDW) method whereby the characterization of sugar composition and consumption of the OPF juice by Ceratocystis fimbriata were carried out using high performance liquid chromatography (HPLC). The volatile organic compounds were separated and analyzed by gas chromatography-mass spectrometry (GC-MS) with solid phase micro extraction (SPME). On the fourth day, the biomass produced by Ceratocystis fimbriata increased significantly and produced the highest number of products comprising of 3 alcohols, 2 esters, 1 aldehyde, 1 fatty acid, 1 phenol and 1 ketone. Based on the GC-MS analysis, several volatile compounds namely aldehydes, alcohols, ketones, and esters could be produced from OPF juice supplemented with a mineral salt medium. This result shows the potential usage of OPF juice as substrates for the growth and production of volatile organic compounds by Ceratocystis fimbriata.

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Abstract: Agarwood has been used for its fragrance and medicinal properties in Asian culture for centuries. In recent years, agarwood gains its popularity in the west due to its usage in perfume formulation. Unfortunately, the supply does not meet the augmentation market demand. This is mostly because of depleting nature agarwood reservoir and lack of induction technique that can produce agarwood consistently in the plantation. In this study, we look into a case where artificial inducing technique successfully produced high quality agarwood. To assure its quality, agarwood chip was analysed by Gas Chromatography for its chemical profiles. Discovered compounds were identified as sesquiterpene group which also had been characterized as major agarwood compound listed on previous studies. Few compounds that are detected such as δ-cadinene (0.20%), jinkoheremol (22.09%), epi-α-cadinol (4.74%), agarospirol (3.75%) and others. Soil condition that contributes into this agarwood formation; soil analysis on physical properties, chemical properties and nutrients content of the soil have been analyzed. Based on the findings, soil condition is an important factor to successfully induced high quality agarwood.